Silver diamine fluoride가 타액 생물막에 미치는 영향

서미경; 송지수; 신터전; 현홍근; 김정욱; 장기택; 이상훈; 김영재

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Silver diamine fluoride가 타액 생물막에 미치는 영향
The Effect of Silver Diamine Fluoride on Salivary Biofilm

대한소아치과학회지 2020년 47권 4호 p.406 ~ 415

서미경, 송지수, 신터전, 현홍근, 김정욱, 장기택, 이상훈, 김영재,

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서미경 ( Seo Mee-Kyung ) - Seoul National University School of Dentistry Department of Pediatric Dentistry
송지수 ( Song Ji-Soo ) - Seoul National University School of Dentistry Department of Pediatric Dentistry
신터전 ( Shin Teo-Jeon ) - Seoul National University School of Dentistry Department of Pediatric Dentistry
현홍근 ( Hyun Hong-Keun ) - Seoul National University School of Dentistry Department of Pediatric Dentistry
김정욱 ( Kim Jung-Wook ) - Seoul National University School of Dentistry Department of Pediatric Dentistry
장기택 ( Jang Ki-Taeg ) - Seoul National University School of Dentistry Department of Pediatric Dentistry
이상훈 ( Lee Sang-Hoon ) - Seoul National University School of Dentistry Department of Pediatric Dentistry
김영재 ( Kim Young-Jae ) - Seoul National University School of Dentistry Department of Pediatric Dentistry

KMID : 0358920200470040406 DOI : 10.5933/JKAPD.2020.47.4.406

Abstract

Silver diamine fluoride(SDF)는 우식의 정지를 위해 사용될 수 있는 효과적이고 경제적인 약제로, 협조가 힘든 환자나 의과적인 문제로 치료를 받기 힘든 경우 등에서 사용될 수 있다. 이 연구는 타액 생물막을 이용하여 SDF 의 항미생물효과를 평가하고자 시행되었다. 타액을 이용하여 획득피막 유사 구조를 형성한 후, 타액과 Streptococcus mutans를 첨가하여 우식원성의 타액 생물막을 형성하였다. 타액 생물막에 SDF 를 처리한 결과, 전체 미생물과 S. mutans의 수가 유의하게 감소하였다(p < 0.000). CLSM에서도 생물막 내 죽은 세포가 증가하였다. 유치 법랑질과 상아질 시편에 SDF를 처리한 후 우식원성의 생물막을 배양한 결과, SDF 를 처리할 경우 생물막 형성이 저해되는 것을 확인할 수 있었다(p = 0.029). 연구 결과 SDF의 뛰어난 항미생물효과를 확인할 수 있었으며, 이로 인한 임상에서의 항우식효과를 기대할 수 있다.

Silver diamine fluoride (SDF) is an effective and efficient agent for arresting dental caries. It can be useful in treating children with behavioral or medical limitations. The purpose of this study was to evaluate the antimicrobial effect of SDF by using salivary biofilm. Pellicle-like saliva coated structure was prepared by using unstimulated saliva. For developing cariogenic biofilm, Streptococcus mutans was added to the mixture of pooled saliva and inoculated into a saliva coated glass or chamber. SDF was applied to cariogenic biofilm to evaluate the antimicrobial effect of SDF. As time passed, total bacteria and S. mutans were reduced after application of SDF (p < 0.000). Confocal laser scanning microscope also showed the increment of the ratio of dead cell. As a result of experiment using enamel and dentin of primary teeth, it was confirmed that the growth of cariogenic biofilm was inhibited when the SDF was treated (p = 0.029 each). This study showed excellent anti-microbial effect of SDF. And anti-caries effect in clinical practice can be expected.